Regulatory

Part:BBa_K2715011:Design

Designed by: Daniel Partridge   Group: iGEM18_Nottingham   (2018-10-08)


Constitutive promoter from C.sporogenes ferrodoxin gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was natively biobrick compatible. The -35 and -10 binding regions were predicted using the BPROM algorithm (see references below), and the promoter region identified.


Source

This basic part is the promoter portion of the ferredoxin regulatory region from the Gram-positive organism Clostridium sporogenes.


References

V. Solovyev, A Salamov (2011) Automatic Annotation of Microbial Genomes and Metagenomic Sequences. In Metagenomics and its Applications in Agriculture, Biomedicine and Environmental Studies (Ed. R.W. Li), Nova Science Publishers, p. 61-78